Hotspot

accD

SSC · 61197-62072 · 876 bp

Back to Ahnfeltia

Pi 0.499

MarkerSeek score 81.70

Primer available yes

Label accD

Feature Metadata

label_name: accD
feature_type: gene
region: SSC
length_bp: 876
variable_sites: 385
pi: 0.499
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.88
markerseek_score: 81.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD_p1	TACCACCACCCCATTTCTCT	TCTGGTTTTGGTGCTGGTTT	50.0	45.0	57.9	58.1	2130 bp	0.5	58.4
accD_p2	TACCACCACCCCATTTCTCT	TTCTGGTTTTGGTGCTGGTT	50.0	45.0	57.9	58.1	2131 bp	0.5	58.4
accD_p3	TACCACCACCCCATTTCTCT	TTTCTGGTTTTGGTGCTGGT	50.0	45.0	57.9	58.1	2132 bp	0.5	58.4
accD_p4	TACCACCACCCCATTTCTCT	CTATTACAGCGGCGTTGCTA	50.0	50.0	57.9	58.2	1835 bp	0.5	58.4
accD_p5	TACCACCACCCCATTTCTCT	GCGGCGTTGCTATTTGTTAG	50.0	50.0	57.9	58.2	1827 bp	0.5	58.1