Hotspot

ycf35-psbA

SSC · 49836-50441 · 606 bp

Back to Ahnfeltia

Pi 0.437

MarkerSeek score 83.20

Primer available yes

Label ycf35-psbA

Feature Metadata

label_name: ycf35-psbA
feature_type: igs
region: SSC
length_bp: 606
variable_sites: 248
pi: 0.437
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 83.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf35-psbA_p1	TGCAAGCAGCTCATATTCGT	AACCAATGTACAGGCGGTTT	45.0	45.0	58.0	58.0	910 bp	0.5	59.7
ycf35-psbA_p2	TGCAAGCAGCTCATATTCGT	ACCAATGTACAGGCGGTTTT	45.0	45.0	58.0	58.0	909 bp	0.5	59.7
ycf35-psbA_p3	TGCAAGCAGCTCATATTCGT	TGTGCCGGAGATACCTAGAG	45.0	55.0	58.0	58.0	1353 bp	0.5	59.6
ycf35-psbA_p4	TGCAAGCAGCTCATATTCGT	TTAGTTCATAGGGGCCACCA	45.0	50.0	58.0	58.0	1159 bp	0.5	59.6
ycf35-psbA_p5	TGCAAGCAGCTCATATTCGT	AATATCAACTGGAGGCGCAG	45.0	50.0	58.0	58.0	996 bp	0.5	59.4