Hotspot

ccdA-trnN(gtt)

SSC · 47132-49131 · 2000 bp

Back to Ahnfeltia

Pi 0.471

MarkerSeek score 78.80

Primer available yes

Label ccdA-trnN(gtt)

Feature Metadata

label_name: ccdA-trnN(gtt)
feature_type: igs
region: SSC
length_bp: 2000
variable_sites: 766
pi: 0.471
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.81
markerseek_score: 78.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccdA-trnN(gtt)_p1	GCTTTAGCTCCGGCATTAGT	ACGAATATGAGCTGCTTGCA	50.0	45.0	58.0	58.0	2548 bp	0.5	59.4
ccdA-trnN(gtt)_p2	GCTTTAGCTCCGGCATTAGT	TCAAATCCTTCCTGGGGAGT	50.0	50.0	58.0	57.9	2054 bp	0.5	59.2
ccdA-trnN(gtt)_p3	GCTTTAGCTCCGGCATTAGT	AGCGTGTCTACCAATTCCAC	50.0	50.0	58.0	57.9	2287 bp	0.5	58.9
ccdA-trnN(gtt)_p4	GCTTTAGCTCCGGCATTAGT	GGTTCAAATCCTTCCTGGGG	50.0	55.0	58.0	58.1	2057 bp	0.5	58.5
ccdA-trnN(gtt)_p5	GCTTTAGCTCCGGCATTAGT	GTAGATTGAATGCGGACGGA	50.0	50.0	58.0	57.8	2351 bp	0.5	57.9