primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
cpeB-cpeA_p1	cpeB-cpeA	1	GCTTCTCAACGCAAAATGGA	AAGGTCAGAACTGGAAGGGA	20	20	45.0	50.0	57.3	57.9	0.0	0.0	0.823129	11810	11906	268-268	1.000000	74.9
cpeB-cpeA_p2	cpeB-cpeA	2	GCTTCTCAACGCAAAATGGA	AGGTCAGAACTGGAAGGGAA	20	20	45.0	50.0	57.3	57.9	0.0	1.9	0.823129	11810	11906	267-267	1.000000	74.9
cpeB-cpeA_p3	cpeB-cpeA	3	AGCTTCTCAACGCAAAATGG	AAGGTCAGAACTGGAAGGGA	20	20	45.0	50.0	57.3	57.9	0.0	0.0	0.830460	11810	11906	269-269	1.000000	74.8
cpeB-cpeA_p4	cpeB-cpeA	4	AGCTTCTCAACGCAAAATGG	AGGTCAGAACTGGAAGGGAA	20	20	45.0	50.0	57.3	57.9	0.0	1.9	0.830460	11810	11906	268-268	1.000000	74.8
cpeB-cpeA_p5	cpeB-cpeA	5	GCTTCTCAACGCAAAATGGA	AGCACGTTGGATATTGCCTT	20	20	45.0	45.0	57.3	57.9	0.0	0.0	0.833496	11810	11906	298-298	1.000000	74.7
thiG_p1	thiG	1	TCGAAACGTAGCACCCAAAA	CTTCCGGGTCCAGGAGATAT	20	20	45.0	55.0	58.0	58.0	0.0	10.9	0.066077	43544	44392	1692-1692	0.500000	59.5
thiG_p2	thiG	2	TCGAAACGTAGCACCCAAAA	CTGGCTGGCCTAACGAATAG	20	20	45.0	55.0	58.0	58.1	0.0	13.1	0.178092	43544	44392	2176-2176	0.500000	58.6
thiG_p3	thiG	3	TCGAAACGTAGCACCCAAAA	GGCTGGCCTAACGAATAGAG	20	20	45.0	55.0	58.0	57.8	0.0	3.3	0.205515	43544	44392	2174-2174	0.500000	58.4
thiG_p4	thiG	4	TCGAAACGTAGCACCCAAAA	ATTGAACTGGCTGGCCTAAC	20	20	45.0	50.0	58.0	58.2	0.0	13.1	0.209748	43544	44392	2182-2182	0.500000	58.3
thiG_p5	thiG	5	TCGAAACGTAGCACCCAAAA	ACCTGAATCAGTTGGGCTTC	20	20	45.0	50.0	58.0	57.8	0.0	9.6	0.255748	43544	44392	1708-1708	0.500000	58.0
ccs1_p1	ccs1	1	ATATCTCCTGGACCCGGAAG	CATACGGATCGATGGACTGC	20	20	55.0	55.0	58.0	58.3	0.7	0.5	0.314018	44980	46287	2198-2198	0.500000	57.5
ccs1_p2	ccs1	2	ACTCAATACTGCAGTAGCGC	CATACGGATCGATGGACTGC	20	20	50.0	55.0	58.1	58.3	29.0	0.5	0.369219	44980	46287	2537-2537	0.500000	57.0
ccs1_p3	ccs1	3	CTCAATACTGCAGTAGCGCA	CATACGGATCGATGGACTGC	20	20	50.0	55.0	58.1	58.3	29.0	0.5	0.370552	44980	46287	2536-2536	0.500000	57.0
ccs1_p4	ccs1	4	ATATCTCCTGGACCCGGAAG	CGGATCGATGGACTGCAAAT	20	20	55.0	50.0	58.0	58.4	0.7	0.5	0.430483	44980	46287	2194-2194	0.500000	56.6
ccs1_p5	ccs1	5	ACTCAATACTGCAGTAGCGC	CGGATCGATGGACTGCAAAT	20	20	50.0	50.0	58.1	58.4	29.0	0.5	0.485683	44980	46287	2533-2533	0.500000	56.1
ccdA_p1	ccdA	1	TGAAACAACTCACCACTGGG	GCTTGCGAAAAATGGTGGAA	20	20	50.0	45.0	57.9	57.9	0.0	0.0	0.198770	46406	47131	2181-2181	0.500000	58.4
ccdA_p2	ccdA	2	ATTTCCAGAATTGTCCGCGA	GCTTGCGAAAAATGGTGGAA	20	20	45.0	45.0	57.9	57.9	8.2	0.0	0.255386	46406	47131	1763-1763	0.500000	58.0
ccdA_p3	ccdA	3	TGAAACAACTCACCACTGGG	GAGCTTGCGAAAAATGGTGG	20	20	50.0	50.0	57.9	58.3	0.0	0.0	0.349630	46406	47131	2183-2183	0.500000	57.2
ccdA_p4	ccdA	4	ATTTCCAGAATTGTCCGCGA	GAGCTTGCGAAAAATGGTGG	20	20	45.0	50.0	57.9	58.3	8.2	0.0	0.406246	46406	47131	1765-1765	0.500000	56.8
ccdA_p5	ccdA	5	TGAAACAACTCACCACTGGG	ATATTTCTTCGGGTGCAGCA	20	20	50.0	45.0	57.9	57.6	0.0	19.1	0.469702	46406	47131	2147-2147	0.500000	56.2
ccdA-trnN(gtt)_p1	ccdA-trnN(gtt)	1	GCTTTAGCTCCGGCATTAGT	ACGAATATGAGCTGCTTGCA	20	20	50.0	45.0	58.0	58.0	0.0	2.5	0.073493	47132	49131	2548-2548	0.500000	59.4
ccdA-trnN(gtt)_p2	ccdA-trnN(gtt)	2	GCTTTAGCTCCGGCATTAGT	TCAAATCCTTCCTGGGGAGT	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.104829	47132	49131	2054-2054	0.500000	59.2
ccdA-trnN(gtt)_p3	ccdA-trnN(gtt)	3	GCTTTAGCTCCGGCATTAGT	AGCGTGTCTACCAATTCCAC	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.139835	47132	49131	2287-2287	0.500000	58.9
ccdA-trnN(gtt)_p4	ccdA-trnN(gtt)	4	GCTTTAGCTCCGGCATTAGT	GGTTCAAATCCTTCCTGGGG	20	20	50.0	55.0	58.0	58.1	0.0	0.0	0.192440	47132	49131	2057-2057	0.500000	58.5
ccdA-trnN(gtt)_p5	ccdA-trnN(gtt)	5	GCTTTAGCTCCGGCATTAGT	GTAGATTGAATGCGGACGGA	20	20	50.0	50.0	58.0	57.8	0.0	0.0	0.264026	47132	49131	2351-2351	0.500000	57.9
ycf35-psbA_p1	ycf35-psbA	1	TGCAAGCAGCTCATATTCGT	AACCAATGTACAGGCGGTTT	20	20	45.0	45.0	58.0	58.0	0.0	16.6	0.038714	49836	50441	910-910	0.500000	59.7
ycf35-psbA_p2	ycf35-psbA	2	TGCAAGCAGCTCATATTCGT	ACCAATGTACAGGCGGTTTT	20	20	45.0	45.0	58.0	58.0	0.0	0.3	0.038714	49836	50441	909-909	0.500000	59.7
ycf35-psbA_p3	ycf35-psbA	3	TGCAAGCAGCTCATATTCGT	TGTGCCGGAGATACCTAGAG	20	20	45.0	55.0	58.0	58.0	0.0	5.0	0.045760	49836	50441	1353-1353	0.500000	59.6
ycf35-psbA_p4	ycf35-psbA	4	TGCAAGCAGCTCATATTCGT	TTAGTTCATAGGGGCCACCA	20	20	45.0	50.0	58.0	58.0	0.0	0.0	0.054392	49836	50441	1159-1159	0.500000	59.6
ycf35-psbA_p5	ycf35-psbA	5	TGCAAGCAGCTCATATTCGT	AATATCAACTGGAGGCGCAG	20	20	45.0	50.0	58.0	58.0	0.0	10.8	0.069378	49836	50441	996-996	0.500000	59.4
rne_p1	rne	1	CTCTAGGTATCTCCGGCACA	ATTATGGGTTGAACCTGGGC	20	20	55.0	50.0	58.0	57.8	0.0	14.9	0.176519	51645	53093	2810-2810	0.500000	58.6
rne_p2	rne	2	CTCTAGGTATCTCCGGCACA	GAGGTCAGATTGTGCTTGCT	20	20	55.0	50.0	58.0	58.2	0.0	0.0	0.198698	51645	53093	2433-2433	0.500000	58.4
rne_p3	rne	3	CTCTAGGTATCTCCGGCACA	TGGAGGTCAGATTGTGCTTG	20	20	55.0	50.0	58.0	57.8	0.0	0.0	0.206934	51645	53093	2435-2435	0.500000	58.3
rne_p4	rne	4	CCCAATTGGTCAAGGAAGCT	ATTATGGGTTGAACCTGGGC	20	20	50.0	50.0	58.1	57.8	22.9	14.9	0.230311	51645	53093	2845-2845	0.500000	58.2
rne_p5	rne	5	CTCTAGTTGCGCCTTCTGTT	ATTATGGGTTGAACCTGGGC	20	20	50.0	50.0	57.9	57.8	4.2	14.9	0.245234	51645	53093	2273-2273	0.500000	58.0
ycf56_p1	ycf56	1	GCCCAGGTTCAACCCATAAT	CATTGCTTACGGGAAGGTCA	20	20	50.0	50.0	57.8	57.9	1.7	1.4	0.267129	53972	54664	1070-1070	0.500000	57.9
ycf56_p2	ycf56	2	GCCCAGGTTCAACCCATAAT	TTGCTTACGGGAAGGTCAAC	20	20	50.0	50.0	57.8	58.1	1.7	1.4	0.272293	53972	54664	1068-1068	0.500000	57.8
ycf56_p3	ycf56	3	AGCAAGCACAATCTGACCTC	CATTGCTTACGGGAAGGTCA	20	20	50.0	50.0	58.2	57.9	0.0	1.4	0.289308	53972	54664	1447-1447	0.500000	57.7
ycf56_p4	ycf56	4	AGCAAGCACAATCTGACCTC	TTGCTTACGGGAAGGTCAAC	20	20	50.0	50.0	58.2	58.1	0.0	1.4	0.294471	53972	54664	1445-1445	0.500000	57.6
ycf56_p5	ycf56	5	CAAGCACAATCTGACCTCCA	CATTGCTTACGGGAAGGTCA	20	20	50.0	50.0	57.8	57.9	0.0	1.4	0.297544	53972	54664	1445-1445	0.500000	57.6
rbcR_p1	rbcR	1	CGAAGTTGCTGTAAAGCTGC	AAGACTTTGCTCTGAAGGCC	20	20	50.0	50.0	58.0	58.1	6.4	0.0	0.141300	55415	56365	1831-1831	0.500000	58.9
rbcR_p2	rbcR	2	TGACCTTCCCGTAAGCAATG	AAGACTTTGCTCTGAAGGCC	20	20	50.0	50.0	57.9	58.1	0.0	0.0	0.205012	55415	56365	2122-2122	0.500000	58.4
rbcR_p3	rbcR	3	GTTGACCTTCCCGTAAGCAA	AAGACTTTGCTCTGAAGGCC	20	20	50.0	50.0	58.1	58.1	0.0	0.0	0.210176	55415	56365	2124-2124	0.500000	58.3
rbcR_p4	rbcR	4	AGCTAGTTGACCTTCCCGTA	AAGACTTTGCTCTGAAGGCC	20	20	50.0	50.0	57.8	58.1	0.0	0.0	0.324024	55415	56365	2129-2129	0.500000	57.4
rbcR_p5	rbcR	5	CGAAGTTGCTGTAAAGCTGC	ACTTTGCTCTGAAGGCCAAT	20	20	50.0	45.0	58.0	57.7	6.4	5.0	0.343564	55415	56365	1828-1828	0.500000	57.3
ycf55_p1	ycf55	1	GCTTAGGTGCTGCTTTTGTC	GTCCAGCCGATTTTATCCCA	20	20	50.0	50.0	57.7	57.7	0.0	0.0	0.679987	56888	57853	2379-2379	0.500000	54.6
ycf55_p2	ycf55	2	GCTTAGGTGCTGCTTTTGTC	TGTCCAGCCGATTTTATCCC	20	20	50.0	50.0	57.7	57.7	0.0	0.0	0.679987	56888	57853	2380-2380	0.500000	54.6
ycf55_p3	ycf55	3	AGGTGCTGCTTTTGTCTCTG	GTCCAGCCGATTTTATCCCA	20	20	50.0	50.0	58.4	57.7	0.0	0.0	0.739536	56888	57853	2375-2375	0.500000	54.1
ycf55_p4	ycf55	4	AGGTGCTGCTTTTGTCTCTG	TGTCCAGCCGATTTTATCCC	20	20	50.0	50.0	58.4	57.7	0.0	0.0	0.739536	56888	57853	2376-2376	0.500000	54.1
ycf55_p5	ycf55	5	TCAATCTGGCTTAGGTGCTG	GTCCAGCCGATTTTATCCCA	20	20	50.0	50.0	57.6	57.7	0.0	0.0	0.750117	56888	57853	2387-2387	0.500000	54.0
ycf53_p1	ycf53	1	TGGCTATTCAGTTCTCGAGG	ACAGAGGCCTTAATCACCCT	20	20	50.0	50.0	56.7	58.0	13.6	6.8	1.284760	57955	58686	1383-1383	0.500000	49.7
ycf53_p2	ycf53	2	TGGCTATTCAGTTCTCGAGG	AGAGGCCTTAATCACCCTGT	20	20	50.0	50.0	56.7	58.0	13.6	6.8	1.284760	57955	58686	1381-1381	0.500000	49.7
ycf53_p3	ycf53	3	GGCTATTCAGTTCTCGAGGT	ACAGAGGCCTTAATCACCCT	20	20	50.0	50.0	56.7	58.0	13.6	6.8	1.286978	57955	58686	1382-1382	0.500000	49.7
ycf53_p4	ycf53	4	GGCTATTCAGTTCTCGAGGT	AGAGGCCTTAATCACCCTGT	20	20	50.0	50.0	56.7	58.0	13.6	6.8	1.286978	57955	58686	1380-1380	0.500000	49.7
ycf53_p5	ycf53	5	TGGCTATTCAGTTCTCGAGG	CACAGAGGCCTTAATCACCC	20	20	50.0	55.0	56.7	58.0	13.6	6.8	1.301470	57955	58686	1384-1384	0.500000	49.6
carA_p1	carA	1	GGGATAAAATCGGCTGGACA	GCATTTGGTGGTCGTTTAGC	20	20	50.0	50.0	57.7	58.0	0.0	0.0	0.364658	58707	59858	1594-1594	0.500000	57.1
carA_p2	carA	2	TGGGATAAAATCGGCTGGAC	GCATTTGGTGGTCGTTTAGC	20	20	50.0	50.0	57.7	58.0	0.0	0.0	0.364658	58707	59858	1595-1595	0.500000	57.1
carA_p3	carA	3	GGGATAAAATCGGCTGGACA	TCTCAGCTAATTGTTCGGCC	20	20	50.0	50.0	57.7	58.0	0.0	0.0	0.366022	58707	59858	1743-1743	0.500000	57.1
carA_p4	carA	4	TGGGATAAAATCGGCTGGAC	TCTCAGCTAATTGTTCGGCC	20	20	50.0	50.0	57.7	58.0	0.0	0.0	0.366022	58707	59858	1744-1744	0.500000	57.1
carA_p5	carA	5	GGGATAAAATCGGCTGGACA	CTCAGCTAATTGTTCGGCCT	20	20	50.0	50.0	57.7	58.0	0.0	0.0	0.371685	58707	59858	1742-1742	0.500000	57.0
accD_p1	accD	1	TACCACCACCCCATTTCTCT	TCTGGTTTTGGTGCTGGTTT	20	20	50.0	45.0	57.9	58.1	0.0	0.0	0.194445	61197	62072	2130-2130	0.500000	58.4
accD_p2	accD	2	TACCACCACCCCATTTCTCT	TTCTGGTTTTGGTGCTGGTT	20	20	50.0	45.0	57.9	58.1	0.0	0.0	0.194445	61197	62072	2131-2131	0.500000	58.4
accD_p3	accD	3	TACCACCACCCCATTTCTCT	TTTCTGGTTTTGGTGCTGGT	20	20	50.0	45.0	57.9	58.1	0.0	0.0	0.194445	61197	62072	2132-2132	0.500000	58.4
accD_p4	accD	4	TACCACCACCCCATTTCTCT	CTATTACAGCGGCGTTGCTA	20	20	50.0	50.0	57.9	58.2	0.0	23.7	0.205929	61197	62072	1835-1835	0.500000	58.4
accD_p5	accD	5	TACCACCACCCCATTTCTCT	GCGGCGTTGCTATTTGTTAG	20	20	50.0	50.0	57.9	58.2	0.0	9.4	0.243550	61197	62072	1827-1827	0.500000	58.1
fabH_p1	fabH	1	AATCCACAGTCCTTCAGGGA	AATGCTGAAGGTGCAAGGAA	20	20	50.0	45.0	58.0	58.0	0.0	0.0	0.045144	62560	63573	2231-2231	0.500000	59.6
fabH_p2	fabH	2	GGGAACACTGTGGACAAACT	AATGCTGAAGGTGCAAGGAA	20	20	50.0	45.0	57.9	58.0	13.1	0.0	0.053134	62560	63573	2307-2307	0.500000	59.6
fabH_p3	fabH	3	AATCCACAGTCCTTCAGGGA	CAACTCTTTGGGTGTTCCGA	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.082999	62560	63573	2094-2094	0.500000	59.3
fabH_p4	fabH	4	GGGAACACTGTGGACAAACT	CAACTCTTTGGGTGTTCCGA	20	20	50.0	50.0	57.9	58.0	13.1	0.0	0.090989	62560	63573	2170-2170	0.500000	59.3
fabH_p5	fabH	5	TAGCAACGCCGCTGTAATAG	AATGCTGAAGGTGCAAGGAA	20	20	50.0	45.0	58.2	58.0	9.3	0.0	0.152534	62560	63573	1906-1906	0.500000	58.8
apcD_p1	apcD	1	TAAATCAGGAACGGCAGAGC	TGATGGCAGATGGACACATG	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.071177	63932	64417	1500-1500	0.500000	59.4
apcD_p2	apcD	2	TGTAGAAGCCAATGCAGCAA	TGATGGCAGATGGACACATG	20	20	45.0	50.0	58.1	58.0	0.0	0.0	0.138658	63932	64417	1635-1635	0.500000	58.9
apcD_p3	apcD	3	TTGTAGAAGCCAATGCAGCA	TGATGGCAGATGGACACATG	20	20	45.0	50.0	58.1	58.0	0.0	0.0	0.138658	63932	64417	1636-1636	0.500000	58.9
apcD_p4	apcD	4	TAAATCAGGAACGGCAGAGC	ACATGGCCATTAGCTGCTTT	20	20	50.0	45.0	58.0	58.1	0.0	17.9	0.167547	63932	64417	1316-1316	0.500000	58.7
apcD_p5	apcD	5	AGAAGCCAATGCAGCAAGAT	TGATGGCAGATGGACACATG	20	20	45.0	50.0	58.2	58.0	0.0	0.0	0.198350	63932	64417	1632-1632	0.500000	58.4
psaF_p1	psaF	1	TTCCTTGCACCTTCAGCATT	CTTTTGCCCAATCCTTTGCC	20	20	45.0	50.0	58.0	58.2	0.0	0.0	0.188117	64743	65318	1730-1730	0.500000	58.5
psaF_p2	psaF	2	TTCCTTGCACCTTCAGCATT	ACCCCATCAGGATCTGTCAT	20	20	45.0	50.0	58.0	57.8	0.0	0.0	0.203472	64743	65318	1853-1853	0.500000	58.4
psaF_p3	psaF	3	TTCCTTGCACCTTCAGCATT	CCCAGATGGTGGCATTGAAT	20	20	45.0	50.0	58.0	58.2	0.0	0.0	0.215994	64743	65318	1684-1684	0.500000	58.3
psaF_p4	psaF	4	TTCCTTGCACCTTCAGCATT	ATCCTTGTCGAGACTCAGGT	20	20	45.0	50.0	58.0	57.8	0.0	16.4	0.224155	64743	65318	1494-1494	0.500000	58.2
psaF_p5	psaF	5	TCGGAACACCCAAAGAGTTG	CTTTTGCCCAATCCTTTGCC	20	20	50.0	50.0	58.0	58.2	0.0	0.0	0.225971	64743	65318	1867-1867	0.500000	58.2
ycf46_p1	ycf46	1	AAGGTCGGAAAAGAATCGGC	GAAGCCCACCTCAAGATGAG	20	20	50.0	55.0	58.5	58.0	0.0	0.3	0.585505	67153	68637	1952-1952	0.500000	55.3
ycf46_p2	ycf46	2	GGTCGGAAAAGAATCGGCTT	GAAGCCCACCTCAAGATGAG	20	20	50.0	55.0	58.5	58.0	0.0	0.3	0.585505	67153	68637	1950-1950	0.500000	55.3
ycf46_p3	ycf46	3	AAGGTCGGAAAAGAATCGGC	TCTCCCCCAAGAGTTCACAT	20	20	50.0	50.0	58.5	58.0	0.0	0.0	0.594768	67153	68637	2256-2256	0.500000	55.2
ycf46_p4	ycf46	4	GGTCGGAAAAGAATCGGCTT	ATCTCCCCCAAGAGTTCACA	20	20	50.0	50.0	58.5	58.0	0.0	0.0	0.594768	67153	68637	2255-2255	0.500000	55.2
ycf46_p5	ycf46	5	GGTCGGAAAAGAATCGGCTT	TCTCCCCCAAGAGTTCACAT	20	20	50.0	50.0	58.5	58.0	0.0	0.0	0.594768	67153	68637	2254-2254	0.500000	55.2