Hotspot

rpl2

IRb · 81088-82572 · 1485 bp

Back to Agrostis

Pi 0.00112

MarkerSeek score 59.50

Primer available yes

Label rpl2

Feature Metadata

label_name: rpl2
feature_type: gene
region: IRb
length_bp: 1485
variable_sites: 3
pi: 0.00112
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl2_p1	GTTCGCCCATCGCATTATTG	CCCTTCAACCGGGTTATTCT	50.0	50.0	58.0	57.2	1615 bp	1.0	73.4
rpl2_p2	TGGATCAAGGCAGTGGATTG	CCCTTCAACCGGGTTATTCT	50.0	50.0	57.9	57.2	1667 bp	1.0	72.7
rpl2_p3	GGATCAAGGCAGTGGATTGT	CCCTTCAACCGGGTTATTCT	50.0	50.0	57.9	57.2	1666 bp	1.0	72.7
rpl2_p4	GTGGATCAAGGCAGTGGATT	CCCTTCAACCGGGTTATTCT	50.0	50.0	57.9	57.2	1668 bp	1.0	72.7
rpl2_p5	GATTGTGAATCCACCATGCG	CCCTTCAACCGGGTTATTCT	50.0	50.0	57.5	57.2	1652 bp	1.0	69.9