Hotspot

rpl16

LSC · 77634-79066 · 1433 bp

Back to Agrostis

Pi 0.00757

MarkerSeek score 53.90

Primer available yes

Label rpl16

Feature Metadata

label_name: rpl16
feature_type: gene
region: LSC
length_bp: 1433
variable_sites: 19
pi: 0.00757
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 53.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16_p1	TCTCAATTCCTCGGCAATCG	CTTCGCATTCTGTCCAGTGA	50.0	50.0	58.1	57.9	1959-2038 bp	1.0	82.4
rpl16_p2	TCTCAATTCCTCGGCAATCG	TCTTCGCATTCTGTCCAGTG	50.0	50.0	58.1	57.9	1960-2039 bp	1.0	82.4
rpl16_p3	TCTCAATTCCTCGGCAATCG	TGTCTTCGCATTCTGTCCAG	50.0	50.0	58.1	57.9	1962-2041 bp	1.0	82.4
rpl16_p4	TCTCAATTCCTCGGCAATCG	GTCTTCGCATTCTGTCCAGT	50.0	50.0	58.1	57.9	1961-2040 bp	1.0	82.4
rpl16_p5	TCGGATCTTTCTAGGGGCAT	CTTCGCATTCTGTCCAGTGA	50.0	50.0	57.9	57.9	1805-1884 bp	1.0	82.3