Hotspot

psbE-petL

LSC · 62231-63517 · 1287 bp

Back to Agrostis

Pi 0.012

MarkerSeek score 61.80

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1287
variable_sites: 29
pi: 0.012
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	TGACAAATAACCAACCCGCA	GCCAATAAATAGAGCTGGGGT	45.0	47.6	58.0	57.8	1472-1480 bp	1.0	71.7
psbE-petL_p2	CAACCCGCAATGAATAGGGA	GCCAATAAATAGAGCTGGGGT	50.0	47.6	57.9	57.8	1461-1469 bp	1.0	71.4
psbE-petL_p3	ACCAACCCGCAATGAATAGG	GCCAATAAATAGAGCTGGGGT	50.0	47.6	58.2	57.8	1463-1471 bp	1.0	70.0
psbE-petL_p4	TGACAAATAACCAACCCGCA	AGCCAATAAATAGAGCTGGGG	45.0	47.6	58.0	57.5	1473-1481 bp	1.0	69.4
psbE-petL_p5	CAACCCGCAATGAATAGGGA	AGCCAATAAATAGAGCTGGGG	50.0	47.6	57.9	57.5	1462-1470 bp	1.0	69.2