Hotspot

rbcL-psaI

LSC · 55767-57317 · 1551 bp

Back to Agrostis

Pi 0.0133
MarkerSeek score 53.00
Primer available yes
Label rbcL-psaI

Feature Metadata

label_name
rbcL-psaI
feature_type
igs
region
LSC
length_bp
1551
variable_sites
39
pi
0.0133
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
53.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rbcL-psaI_p1 CATAACTGGGGGATTCACCG ATACCCGCATCCATCCCATA 55.0 50.0 58.0 58.0 2397-2413 bp 1.0 81.1
rbcL-psaI_p2 CATAACTGGGGGATTCACCG TATACCCGCATCCATCCCAT 55.0 50.0 58.0 58.0 2398-2414 bp 1.0 81.1
rbcL-psaI_p3 TCTTCACATTCACCGTGCAA ATACCCGCATCCATCCCATA 45.0 50.0 58.0 58.0 2334-2350 bp 1.0 81.1
rbcL-psaI_p4 TCTTCACATTCACCGTGCAA TATACCCGCATCCATCCCAT 45.0 50.0 58.0 58.0 2335-2351 bp 1.0 81.1
rbcL-psaI_p5 CATAACTGGGGGATTCACCG CACGACTCCCTGTGGAAAAA 55.0 50.0 58.0 58.0 2817-2837 bp 1.0 80.9