Hotspot

ndhC-trnV-UAC

LSC · 49667-50581 · 915 bp

Back to Agrostis

Pi 0.0151
MarkerSeek score 62.90
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
915
variable_sites
24
pi
0.0151
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.99
markerseek_score
62.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 CCCTGAAATCCAAAATGCCA GGTTCGAATCCGTATAGCCC 45.0 55.0 56.6 57.9 1002-1034 bp 1.0 71.5
ndhC-trnV-UAC_p2 CCCTGAAATCCAAAATGCCA CGGTTCGAATCCGTATAGCC 45.0 55.0 56.6 58.3 1003-1035 bp 1.0 69.7
ndhC-trnV-UAC_p3 CCCTGAAATCCAAAATGCCAA GGTTCGAATCCGTATAGCCC 42.9 55.0 57.3 57.9 1002-1034 bp 1.0 69.5
ndhC-trnV-UAC_p4 CCCTGAAATCCAAAATGCCAA CGGTTCGAATCCGTATAGCC 42.9 55.0 57.3 58.3 1003-1035 bp 1.0 67.7
ndhC-trnV-UAC_p5 CCCTGAAATCCAAAATGCCAAA GGTTCGAATCCGTATAGCCC 40.9 55.0 58.0 57.9 1002-1034 bp 1.0 66.9