Hotspot

trnF-GAA-ndhJ

LSC · 47419-47997 · 579 bp

Back to Agrostis

Pi 0.0115

MarkerSeek score 61.50

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 579
variable_sites: 12
pi: 0.0115
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	CCTCGTGTCACCAGTTCAAA	TGAAAGTTGGATAGGCTGGC	50.0	50.0	58.1	57.9	689-697 bp	1.0	80.1
trnF-GAA-ndhJ_p2	CCTCGTGTCACCAGTTCAAA	CGCGCCTTAAACGTATCCTA	50.0	50.0	58.1	57.9	714-722 bp	1.0	80.0
trnF-GAA-ndhJ_p3	CCTCGTGTCACCAGTTCAAA	ATAGGCTGGCCCTTACGTAA	50.0	50.0	58.1	58.2	679-687 bp	1.0	79.5
trnF-GAA-ndhJ_p4	CCTCGTGTCACCAGTTCAAA	ATGATAATCATCCGCGCCTT	50.0	45.0	58.1	57.5	726-734 bp	1.0	77.2
trnF-GAA-ndhJ_p5	CCTCGTGTCACCAGTTCAAA	ATCCGCGCCTTAAACGTATC	50.0	50.0	58.1	58.5	717-725 bp	1.0	77.0