Hotspot

trnC-GCA-rpoB

LSC · 18243-19405 · 1163 bp

Back to Agrostis

Pi 0.00931

MarkerSeek score 59.20

Primer available yes

Label trnC-GCA-rpoB

Feature Metadata

label_name: trnC-GCA-rpoB
feature_type: igs
region: LSC
length_bp: 1163
variable_sites: 19
pi: 0.00931
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-rpoB_p1	AAAGGATTTGCAGTCCCCTG	TGTGGACATTCCCTCGTTTC	50.0	50.0	58.1	57.8	1239-1248 bp	1.0	79.5
trnC-GCA-rpoB_p2	AAAGGATTTGCAGTCCCCTG	TTCCCTCGTTTCCATTTCGG	50.0	50.0	58.1	58.2	1231-1240 bp	1.0	79.4
trnC-GCA-rpoB_p3	AAAGGATTTGCAGTCCCCTG	CCGGGTATTGTGGACATTCC	50.0	55.0	58.1	58.3	1247-1256 bp	1.0	78.3
trnC-GCA-rpoB_p4	AAAGGATTTGCAGTCCCCTG	CGGGTATTGTGGACATTCCC	50.0	55.0	58.1	58.3	1246-1255 bp	1.0	78.3
trnC-GCA-rpoB_p5	AAAGGATTTGCAGTCCCCTG	AAGTTCTTCTGCCAAGGCTT	50.0	45.0	58.1	57.6	1311-1320 bp	1.0	77.9