Hotspot

petN-trnC-GCA

LSC · 17263-18171 · 909 bp

Back to Agrostis

Pi 0.0109

MarkerSeek score 60.60

Primer available yes

Label petN-trnC-GCA

Feature Metadata

label_name: petN-trnC-GCA
feature_type: igs
region: LSC
length_bp: 909
variable_sites: 18
pi: 0.0109
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 60.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-trnC-GCA_p1	AGCCCAAGCGAGACTTACTA	ACGTGGACGAATACTTGCTC	50.0	50.0	58.2	58.0	1045-1065 bp	1.0	81.2
petN-trnC-GCA_p2	CCACTCCTCCCCCATACTAC	ACGTGGACGAATACTTGCTC	60.0	50.0	58.3	58.0	1101-1121 bp	1.0	80.1
petN-trnC-GCA_p3	TCCACTCCTCCCCCATACTA	ACGTGGACGAATACTTGCTC	55.0	50.0	58.4	58.0	1102-1122 bp	1.0	79.3
petN-trnC-GCA_p4	AGCCCAAGCGAGACTTACTA	CGTGGACGAATACTTGCTCT	50.0	50.0	58.2	57.7	1044-1064 bp	1.0	79.1
petN-trnC-GCA_p5	CCACTCCTCCCCCATACTAC	CGTGGACGAATACTTGCTCT	60.0	50.0	58.3	57.7	1100-1120 bp	1.0	77.9