Hotspot

rps16

LSC · 4511-5579 · 1069 bp

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Pi 0.000624

MarkerSeek score 46.80

Primer available yes

Label rps16

Feature Metadata

label_name: rps16
feature_type: gene
region: LSC
length_bp: 1069
variable_sites: 2
pi: 0.000624
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 46.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16_p1	TGGCACGATATAAATCCAAATAGG	TGCTCCATAGATAGCAAGTT	37.5	40.0	56.9	53.8	1288-1295 bp	1.0	40.1
rps16_p2	TGGCACGATATAAATCCAAATAGG	TCCATATTGCTCCATAGATAGCA	37.5	39.1	56.9	56.7	1295-1302 bp	1.0	40.1
rps16_p3	TGGCACGATATAAATCCAAATAGG	TGCTCCATAGATAGCAAGTTT	37.5	38.1	56.9	54.7	1288-1295 bp	1.0	40.1
rps16_p4	TGGCACGATATAAATCCAAA	TGCTCCATAGATAGCAAGTT	35.0	40.0	52.6	53.8	1288-1295 bp	1.0	40.1
rps16_p5	TTGGCACGATATAAATCCAA	TGCTCCATAGATAGCAAGTT	35.0	40.0	52.6	53.8	1289-1296 bp	1.0	40.1