Hotspot

rpl16

LSC · 76776-78233 · 1458 bp

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Pi 0.00128

MarkerSeek score 45.40

Primer available yes

Label rpl16

Feature Metadata

label_name: rpl16
feature_type: gene
region: LSC
length_bp: 1458
variable_sites: 5
pi: 0.00128
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 45.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16_p1	TCCAGAAAGAAAAACCGGGC	TCCTGAAATCCCTGCAAAAGA	50.0	42.9	58.7	57.5	1593-1595 bp	1.0	62.7
rpl16_p2	CCAGAAAGAAAAACCGGGCA	TCCTGAAATCCCTGCAAAAGA	50.0	42.9	59.0	57.5	1592-1594 bp	1.0	60.4
rpl16_p3	CAGAAAGAAAAACCGGGCAT	TCCTGAAATCCCTGCAAAAGA	45.0	42.9	56.9	57.5	1591-1593 bp	1.0	59.3
rpl16_p4	TCCAGAAAGAAAAACCGGGC	CCTGAAATCCCTGCAAAAGA	50.0	45.0	58.7	55.9	1592-1594 bp	1.0	58.2
rpl16_p5	TCCAGAAAGAAAAACCGGGC	TCCTGAAATCCCTGCAAAAG	50.0	45.0	58.7	55.9	1593-1595 bp	1.0	58.2