Hotspot

psbA

LSC · 92-1153 · 1062 bp

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Pi 0.000314

MarkerSeek score 33.30

Primer available yes

Label psbA

Feature Metadata

label_name: psbA
feature_type: gene
region: LSC
length_bp: 1062
variable_sites: 1
pi: 0.000314
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 33.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbA_p1	AGCAAGATATTGGGTATTTCTGTCT	AATACGTGTGCTTGGGAGTC	36.0	50.0	58.1	57.9	1177 bp	1.0	40.1
psbA_p2	AGCAAGATATTGGGTATTTCTGTC	AATACGTGTGCTTGGGAGTC	37.5	50.0	56.8	57.9	1177 bp	1.0	40.1
psbA_p3	GCAAGATATTGGGTATTTCTGTCT	AATACGTGTGCTTGGGAGTC	37.5	50.0	56.8	57.9	1176 bp	1.0	40.1
psbA_p4	AGCAAGATATTGGGTATTTCTGT	AATACGTGTGCTTGGGAGTC	34.8	50.0	55.7	57.9	1177 bp	1.0	40.1
psbA_p5	GCAAGATATTGGGTATTTCTGTC	AATACGTGTGCTTGGGAGTC	39.1	50.0	55.4	57.9	1176 bp	1.0	40.1