Hotspot

atpF

LSC · 32897-34275 · 1379 bp

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Pi 0

MarkerSeek score 50.50

Primer available yes

Label atpF

Feature Metadata

label_name: atpF
feature_type: gene
region: LSC
length_bp: 1379
variable_sites: 0
pi: 0
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 50.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF_p1	ACGAGCGAAGTAAGTGATCG	TTCGGAAGCAAGGGGAAAAA	50.0	45.0	57.8	57.9	1508-1515 bp	1.0	78.7
atpF_p2	ACGAGCGAAGTAAGTGATCG	TTTCGGAAGCAAGGGGAAAA	50.0	45.0	57.8	57.9	1509-1516 bp	1.0	78.7
atpF_p3	ACGAGCGAAGTAAGTGATCG	TTTTCGGAAGCAAGGGGAAA	50.0	45.0	57.8	57.9	1510-1517 bp	1.0	78.7
atpF_p4	ACGAGCGAAGTAAGTGATCG	TTTTTCGGAAGCAAGGGGAA	50.0	45.0	57.8	57.9	1511-1518 bp	1.0	78.7
atpF_p5	ACGAGCGAAGTAAGTGATCG	TCGAAGGGTTGCCATTAGTG	50.0	50.0	57.8	57.9	1543-1550 bp	1.0	78.4