Hotspot

rpl32-trnL-UAG

SSC · 104543-105243 · 701 bp

Back to Agropyron

Pi 0.000951

MarkerSeek score 47.30

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 701
variable_sites: 2
pi: 0.000951
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 47.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	TTCCAGCGGTAATGAGCATC	TTCGAACCGAGATGCTTGAG	50.0	50.0	58.0	58.0	838-842 bp	1.0	80.0
rpl32-trnL-UAG_p2	TTCCAGCGGTAATGAGCATC	GGATTCGAACCGAGATGCTT	50.0	50.0	58.0	58.1	841-845 bp	1.0	79.5
rpl32-trnL-UAG_p3	AAGGGTTTTTCTGGGCAACA	TTCGAACCGAGATGCTTGAG	45.0	50.0	58.1	58.0	810-814 bp	1.0	79.3
rpl32-trnL-UAG_p4	AGGGTTTTTCTGGGCAACAA	TTCGAACCGAGATGCTTGAG	45.0	50.0	58.1	58.0	809-813 bp	1.0	79.3
rpl32-trnL-UAG_p5	TTCCAGCGGTAATGAGCATC	TCCTAAGAGCAGCGTGTCTA	50.0	50.0	58.0	57.9	811-815 bp	1.0	79.0