Hotspot

rps15-ndhF

IRb · 100868-101219 · 352 bp

Back to Agropyron

Pi 0.0053

MarkerSeek score 43.10

Primer available yes

Label rps15-ndhF

Feature Metadata

label_name: rps15-ndhF
feature_type: igs
region: IRb
length_bp: 352
variable_sites: 5
pi: 0.0053
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 43.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15-ndhF_p1	CTTTTGGGAAAACGTCGACG	ATGTAGGGGGAGGACGAATC	50.0	55.0	58.0	58.3	531-534 bp	1.0	78.8
rps15-ndhF_p2	TCGACGTTTGCTGGCTTATT	ATGTAGGGGGAGGACGAATC	45.0	55.0	58.2	58.3	517-520 bp	1.0	77.4
rps15-ndhF_p3	CTTTTGGGAAAACGTCGACG	GTAGGGGGAGGACGAATCTC	50.0	60.0	58.0	58.7	529-532 bp	1.0	75.7
rps15-ndhF_p4	GTTTGCTGGCTTATTTGGCA	ATGTAGGGGGAGGACGAATC	45.0	55.0	57.5	58.3	512-515 bp	1.0	75.4
rps15-ndhF_p5	TCGACGTTTGCTGGCTTATT	GTAGGGGGAGGACGAATCTC	45.0	60.0	58.2	58.7	515-518 bp	1.0	74.3