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rbcL-psaI

LSC · 56049-56908 · 860 bp

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Pi 0.00482

MarkerSeek score 49.60

Primer available yes

Label rbcL-psaI

Feature Metadata

label_name: rbcL-psaI
feature_type: igs
region: LSC
length_bp: 860
variable_sites: 7
pi: 0.00482
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 49.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-psaI_p1	CGCAGCTTGTGAAGTATGGA	GTCATTGCAATTGCCGGAAA	50.0	45.0	58.3	57.9	996-998 bp	1.0	77.9
rbcL-psaI_p2	AGTTCGAGCCGGTAGATACT	GTCATTGCAATTGCCGGAAA	50.0	45.0	57.7	57.9	961-963 bp	1.0	77.4
rbcL-psaI_p3	GGCGATCAAATTCGAGTTCG	GTCATTGCAATTGCCGGAAA	50.0	45.0	57.6	57.9	975-977 bp	1.0	77.3
rbcL-psaI_p4	TGGAAGGCGATCAAATTCGA	GTCATTGCAATTGCCGGAAA	45.0	45.0	57.6	57.9	980-982 bp	1.0	77.0
rbcL-psaI_p5	AGCTTGCAAATGGAGTCCTG	GTCATTGCAATTGCCGGAAA	50.0	45.0	58.5	57.9	1023-1025 bp	1.0	76.4