Hotspot

petN-trnC-GCA

LSC · 17455-18402 · 948 bp

Back to Agropyron

Pi 0.00183

MarkerSeek score 60.20

Primer available yes

Label petN-trnC-GCA

Feature Metadata

label_name: petN-trnC-GCA
feature_type: igs
region: LSC
length_bp: 948
variable_sites: 4
pi: 0.00183
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-trnC-GCA_p1	AGTCCACTCCTTCCCCATAC	AGTTCAAATCTGGGTGCCG	55.0	52.6	58.1	58.1	1055-1057 bp	1.0	71.0
petN-trnC-GCA_p2	GTCCACTCCTTCCCCATACT	AGTTCAAATCTGGGTGCCG	55.0	52.6	58.1	58.1	1054-1056 bp	1.0	71.0
petN-trnC-GCA_p3	AGCCCAAGCGAGACTTACTA	AGTTCAAATCTGGGTGCCG	50.0	52.6	58.2	58.1	996-998 bp	1.0	70.8
petN-trnC-GCA_p4	AGTCCACTCCTTCCCCATAC	CCAGTTCAAATCTGGGTGCC	55.0	55.0	58.1	59.1	1057-1059 bp	1.0	70.6
petN-trnC-GCA_p5	GTCCACTCCTTCCCCATACT	CCAGTTCAAATCTGGGTGCC	55.0	55.0	58.1	59.1	1056-1058 bp	1.0	70.6