Hotspot

trnG-GCC-trnM-CAU

LSC · 13534-14648 · 1115 bp

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Pi 0.00108
MarkerSeek score 50.20
Primer available yes
Label trnG-GCC-trnM-CAU

Feature Metadata

label_name
trnG-GCC-trnM-CAU
feature_type
igs
region
LSC
length_bp
1115
variable_sites
3
pi
0.00108
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
50.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnG-GCC-trnM-CAU_p1 ACGAATCACACTTTTACCACT TGAACCGATGACTTATGCCT 38.1 45.0 55.5 56.6 1199 bp 1.0 40.9
trnG-GCC-trnM-CAU_p2 ACGAATCACACTTTTACCACT TGACTTACGTCTTAGCACGAG 38.1 47.6 55.5 57.5 1259 bp 1.0 40.2
trnG-GCC-trnM-CAU_p3 ACGAATCACACTTTTACCACT CCGATGACTTATGCCTTACCA 38.1 47.6 55.5 57.5 1195 bp 1.0 40.2
trnG-GCC-trnM-CAU_p4 ACGAATCACACTTTTACCACT ACCGATGACTTATGCCTTACC 38.1 47.6 55.5 57.5 1196 bp 1.0 40.2
trnG-GCC-trnM-CAU_p5 GAACGAATCACACTTTTACCACT TGAACCGATGACTTATGCCT 39.1 45.0 57.3 56.6 1201 bp 1.0 40.2