Hotspot

trnS-UGA-psbZ

LSC · 11274-11630 · 357 bp

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Pi 0.000934

MarkerSeek score 38.20

Primer available yes

Label trnS-UGA-psbZ

Feature Metadata

label_name: trnS-UGA-psbZ
feature_type: igs
region: LSC
length_bp: 357
variable_sites: 1
pi: 0.000934
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 38.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-UGA-psbZ_p1	CAACCACTCAGCCATCTCTC	CCAACCATCAGGAGAAGCAA	55.0	50.0	58.0	57.8	477 bp	1.0	78.4
trnS-UGA-psbZ_p2	GAGCTATCAACCACTCAGCC	CCAACCATCAGGAGAAGCAA	55.0	50.0	58.1	57.8	484 bp	1.0	78.1
trnS-UGA-psbZ_p3	GGAGCTATCAACCACTCAGC	CCAACCATCAGGAGAAGCAA	55.0	50.0	58.1	57.8	485 bp	1.0	78.1
trnS-UGA-psbZ_p4	CAAGACCGGAGCTATCAACC	CCAACCATCAGGAGAAGCAA	55.0	50.0	58.1	57.8	492 bp	1.0	78.1
trnS-UGA-psbZ_p5	CAACCACTCAGCCATCTCTC	GACCAACCATCAGGAGAAGC	55.0	55.0	58.0	58.3	479 bp	1.0	77.9