Hotspot

rpl32-trnL-UAG

SSC · 112218-113334 · 1117 bp

Back to Agriophyllum

Pi 0.0271
MarkerSeek score 60.80
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
1117
variable_sites
30
pi
0.0271
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
60.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 GGGATATTGGGCAGCGTTAA TACCATTTCACCATAGCGGC 50.0 50.0 58.0 58.0 1222-1228 bp 1.0 82.3
rpl32-trnL-UAG_p2 GGGATATTGGGCAGCGTTAA CTCGCACTGCTTCCTAAGAG 50.0 55.0 58.0 58.1 1251-1257 bp 1.0 81.9
rpl32-trnL-UAG_p3 GGGATATTGGGCAGCGTTAA CTCTCGCACTGCTTCCTAAG 50.0 55.0 58.0 58.1 1253-1259 bp 1.0 81.9
rpl32-trnL-UAG_p4 GGGATATTGGGCAGCGTTAA ATCCGCATAAAAGAGCTGCA 50.0 45.0 58.0 58.0 1903-1904 bp 1.0 81.8
rpl32-trnL-UAG_p5 GGGATATTGGGCAGCGTTAA TCCGCATAAAAGAGCTGCAT 50.0 45.0 58.0 58.0 1902-1903 bp 1.0 81.8