Hotspot

ndhF-rpl32

SSC · 110927-112058 · 1132 bp

Back to Agriophyllum

Pi 0.0256

MarkerSeek score 72.90

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 1132
variable_sites: 28
pi: 0.0256
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 72.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	TGGAAGTGGAAGGAAGGGTA	TTAACGCTGCCCAATATCCC	50.0	50.0	57.6	58.0	1255-1271 bp	1.0	80.5
ndhF-rpl32_p2	TGGAAGTGGAAGGAAGGGTA	AAAAGGCTTTTAACGCTGCC	50.0	45.0	57.6	57.9	1264-1280 bp	1.0	79.5
ndhF-rpl32_p3	TGGAAGTGGAAGGAAGGGTA	CTGCCCAATATCCCCCTTTT	50.0	50.0	57.6	57.5	1249-1265 bp	1.0	76.9
ndhF-rpl32_p4	CTGGAAGTGGAAGGAAGGGT	TTAACGCTGCCCAATATCCC	55.0	50.0	58.9	58.0	1256-1272 bp	1.0	76.5
ndhF-rpl32_p5	GGAAGGGTATGATCCATGCA	TTAACGCTGCCCAATATCCC	50.0	50.0	57.0	58.0	1244-1260 bp	1.0	76.3