Hotspot

rpl16-rps3

LSC · 83786-83935 · 150 bp

Back to Agriophyllum

Pi 0.0333

MarkerSeek score 69.30

Primer available yes

Label rpl16-rps3

Feature Metadata

label_name: rpl16-rps3
feature_type: igs
region: LSC
length_bp: 150
variable_sites: 5
pi: 0.0333
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 69.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16-rps3_p1	AAAAACCGACGAGTCACACA	ACAAACCATTCGCGCAAAAA	45.0	40.0	58.0	58.1	278-279 bp	1.0	80.8
rpl16-rps3_p2	CCGACGAGTCACACACTAAG	ACAAACCATTCGCGCAAAAA	55.0	40.0	58.0	58.1	273-274 bp	1.0	80.6
rpl16-rps3_p3	TGATGCGATGGGTTGGTTAG	ACAAACCATTCGCGCAAAAA	50.0	40.0	58.0	58.1	355 bp	1.0	80.5
rpl16-rps3_p4	GATGCGATGGGTTGGTTAGT	ACAAACCATTCGCGCAAAAA	50.0	40.0	58.0	58.1	354 bp	1.0	80.5
rpl16-rps3_p5	AAAAACCGACGAGTCACACA	TTCCCCTACAAACCATTCGC	45.0	50.0	58.0	58.2	285-286 bp	1.0	79.9