Hotspot

petG-trnW-CCA

LSC · 67328-67462 · 135 bp

Back to Agriophyllum

Pi 0.0677

MarkerSeek score 77.30

Primer available yes

Label petG-trnW-CCA

Feature Metadata

label_name: petG-trnW-CCA
feature_type: igs
region: LSC
length_bp: 135
variable_sites: 9
pi: 0.0677
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 77.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petG-trnW-CCA_p1	ACAGACGTGGTGATCAGTTG	AGAACGTGGGTCTCCAAAAC	50.0	50.0	57.8	58.0	217-219 bp	1.0	82.5
petG-trnW-CCA_p2	ACAGACGTGGTGATCAGTTG	CAAAACCCGATGTCGTAGGT	50.0	50.0	57.8	57.9	203-205 bp	1.0	82.4
petG-trnW-CCA_p3	ACAGACGTGGTGATCAGTTG	AGCGCTCTTAGTTCAGTTCG	50.0	50.0	57.8	58.0	239-241 bp	1.0	82.3
petG-trnW-CCA_p4	GACGTGGTGATCAGTTGGAT	AGAACGTGGGTCTCCAAAAC	50.0	50.0	57.6	58.0	214-216 bp	1.0	80.7
petG-trnW-CCA_p5	GACGTGGTGATCAGTTGGAT	AGCGCTCTTAGTTCAGTTCG	50.0	50.0	57.6	58.0	236-238 bp	1.0	80.5