Hotspot

psbE-petL

LSC · 65682-66972 · 1291 bp

Back to Agriophyllum

Pi 0.0196

MarkerSeek score 69.90

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1291
variable_sites: 25
pi: 0.0196
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 69.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	CAACCCGCAATGAATAGGGA	AGCGGAGGTTATAGTTAAAGCC	50.0	45.5	57.9	57.9	1455-1472 bp	1.0	65.1
psbE-petL_p2	CAACCCGCAATGAATAGGGA	GGAGGTTATAGTTAAAGCCGCT	50.0	45.5	57.9	57.9	1452-1469 bp	1.0	65.1
psbE-petL_p3	ACTGACGAATAACCAACCCG	AGCGGAGGTTATAGTTAAAGCC	50.0	45.5	57.9	57.9	1468-1485 bp	1.0	64.8
psbE-petL_p4	ACTGACGAATAACCAACCCG	GGAGGTTATAGTTAAAGCCGCT	50.0	45.5	57.9	57.9	1465-1482 bp	1.0	64.8
psbE-petL_p5	AGAACGTTCTCCTGTGCTTC	GGAGGTTATAGTTAAAGCCGCT	50.0	45.5	57.8	57.9	1375-1392 bp	1.0	64.4