Hotspot

petA-psbJ

LSC · 63898-64875 · 978 bp

Back to Agriophyllum

Pi 0.0175

MarkerSeek score 69.70

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 978
variable_sites: 17
pi: 0.0175
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 69.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	TGCGCGTACAAGGTCTTTT	CTGGATTAGGCTCATCGCTG	47.4	55.0	58.0	58.2	1113-1114 bp	1.0	72.2
petA-psbJ_p2	CGCGTACAAGGTCTTTTGTT	CTGGATTAGGCTCATCGCTG	45.0	55.0	57.0	58.2	1111-1112 bp	1.0	72.0
petA-psbJ_p3	TGCGCGTACAAGGTCTTTT	TCTGGATTAGGCTCATCGCT	47.4	50.0	58.0	58.3	1114-1115 bp	1.0	71.5
petA-psbJ_p4	CGCGTACAAGGTCTTTTGTTC	CTGGATTAGGCTCATCGCTG	47.6	55.0	58.1	58.2	1111-1112 bp	1.0	71.4
petA-psbJ_p5	CGCGTACAAGGTCTTTTGTT	TCTGGATTAGGCTCATCGCT	45.0	50.0	57.0	58.3	1112-1113 bp	1.0	71.3