Hotspot

ycf4-cemA

LSC · 61063-61956 · 894 bp

Back to Agriophyllum

Pi 0.0289

MarkerSeek score 64.20

Primer available yes

Label ycf4-cemA

Feature Metadata

label_name: ycf4-cemA
feature_type: igs
region: LSC
length_bp: 894
variable_sites: 25
pi: 0.0289
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 64.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf4-cemA_p1	GGAAATCAGAGGCCAAGGAG	AGATGCGTTTCCGGGTATTC	55.0	50.0	58.0	58.1	1234-1260 bp	1.0	82.9
ycf4-cemA_p2	TTGGCCTATTTCTTGCGTGT	AGATGCGTTTCCGGGTATTC	45.0	50.0	58.1	58.1	1149-1175 bp	1.0	82.7
ycf4-cemA_p3	ATGAGAATTTGACGCCACGA	AGATGCGTTTCCGGGTATTC	45.0	50.0	57.9	58.1	1193-1219 bp	1.0	82.6
ycf4-cemA_p4	GAGCCATTCCTTTGACTCGT	AGATGCGTTTCCGGGTATTC	50.0	50.0	57.9	58.1	1217-1243 bp	1.0	82.5
ycf4-cemA_p5	TGAATTGGCGATCAGAACGT	AGATGCGTTTCCGGGTATTC	45.0	50.0	57.9	58.1	1664-1690 bp	1.0	81.7