Hotspot

accD-psaI

LSC · 59364-60052 · 689 bp

Back to Agriophyllum

Pi 0.044

MarkerSeek score 70.00

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 689
variable_sites: 25
pi: 0.044
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.82
markerseek_score: 70.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	AACGCCTACATTGCATTTGC	CCATTGCAATTGCCGGAAAT	45.0	45.0	58.0	58.0	862-957 bp	1.0	86.2
accD-psaI_p2	GCATGCAAGAAGGAAGCTTG	CCATTGCAATTGCCGGAAAT	50.0	45.0	58.0	58.0	1032-1127 bp	1.0	85.8
accD-psaI_p3	AACGCCTACATTGCATTTGC	ACGTTCTGATCGCCAATTCA	45.0	45.0	58.0	57.9	1272-1372 bp	1.0	85.5
accD-psaI_p4	AACGCCTACATTGCATTTGC	TTATGTTCGGAAGAGCGTGG	45.0	50.0	58.0	58.0	1095 bp	0.5	59.9
accD-psaI_p5	GCATGCAAGAAGGAAGCTTG	TTATGTTCGGAAGAGCGTGG	50.0	50.0	58.0	58.0	1265 bp	0.5	59.9