Hotspot

trnG-GCC-trnfM-CAU

LSC · 36377-36586 · 210 bp

Back to Agriophyllum

Pi 0.0585
MarkerSeek score 84.40
Primer available yes
Label trnG-GCC-trnfM-CAU

Feature Metadata

label_name
trnG-GCC-trnfM-CAU
feature_type
igs
region
LSC
length_bp
210
variable_sites
12
pi
0.0585
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
84.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnG-GCC-trnfM-CAU_p1 TCTTTGCCAAGGAGAAGACG CTTGAGGTCACGGGTTCAAA 50.0 50.0 57.8 58.0 288-293 bp 1.0 83.0
trnG-GCC-trnfM-CAU_p2 TCTTTGCCAAGGAGAAGACG GTCACGGGTTCAAATCCTGT 50.0 50.0 57.8 58.1 282-287 bp 1.0 82.6
trnG-GCC-trnfM-CAU_p3 GCGGGGACATATTCCTTCTC CTTGAGGTCACGGGTTCAAA 55.0 50.0 57.8 58.0 348-353 bp 1.0 82.2
trnG-GCC-trnfM-CAU_p4 TCTTTGCCAAGGAGAAGACG TCACGGGTTCAAATCCTGTC 50.0 50.0 57.8 57.8 281-286 bp 1.0 82.0
trnG-GCC-trnfM-CAU_p5 GCGGGGACATATTCCTTCTC GTCACGGGTTCAAATCCTGT 55.0 50.0 57.8 58.1 342-347 bp 1.0 81.7