Hotspot

psbC-trnS-UGA

LSC · 35089-35323 · 235 bp

Back to Agriophyllum

Pi 0.0693
MarkerSeek score 72.00
Primer available yes
Label psbC-trnS-UGA

Feature Metadata

label_name
psbC-trnS-UGA
feature_type
igs
region
LSC
length_bp
235
variable_sites
16
pi
0.0693
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
72.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbC-trnS-UGA_p1 TCTTTCGATGACCCCTCTTA GAGAGATGGCTGAGTGGTTG 45.0 55.0 55.3 58.0 349-352 bp 1.0 62.6
psbC-trnS-UGA_p2 TCTTTCGATGACCCCTCTTA GGTTGATAGCTCCGGTCTTG 45.0 55.0 55.3 58.1 334-337 bp 1.0 62.5
psbC-trnS-UGA_p3 TCTTTCGATGACCCCTCTTA GCTGAGTGGTTGATAGCTCC 45.0 55.0 55.3 58.1 341-344 bp 1.0 62.4
psbC-trnS-UGA_p4 TCTTTCGATGACCCCTCTTA GGCTGAGTGGTTGATAGCTC 45.0 55.0 55.3 58.1 342-345 bp 1.0 62.4
psbC-trnS-UGA_p5 TCGATGACCCCTCTTAATTGA GAGAGATGGCTGAGTGGTTG 42.9 55.0 56.2 58.0 345-348 bp 1.0 61.7