Hotspot

trnD-GUC-trnY-GUA

LSC · 30250-30699 · 450 bp

Back to Agriophyllum

Pi 0.0522

MarkerSeek score 77.80

Primer available yes

Label trnD-GUC-trnY-GUA

Feature Metadata

label_name: trnD-GUC-trnY-GUA
feature_type: igs
region: LSC
length_bp: 450
variable_sites: 23
pi: 0.0522
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 77.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnD-GUC-trnY-GUA_p1	GCGGTGCTCTAACCAATTGA	TGGCAATATGTCTACGCTGG	50.0	50.0	58.6	57.8	521-522 bp	1.0	78.6
trnD-GUC-trnY-GUA_p2	GCGGTGCTCTAACCAATTGA	GGCAATATGTCTACGCTGGT	50.0	50.0	58.6	57.8	520-521 bp	1.0	78.6
trnD-GUC-trnY-GUA_p3	GCGGTGCTCTAACCAATTGA	GGGGACGGACTGTAAATTCG	50.0	55.0	58.6	58.4	542-543 bp	1.0	77.5
trnD-GUC-trnY-GUA_p4	GCGGTGCTCTAACCAATTGA	GGGACGGACTGTAAATTCGT	50.0	50.0	58.6	57.6	541-542 bp	1.0	77.3
trnD-GUC-trnY-GUA_p5	TTGGATTGATCGGGACTGAC	TGGCAATATGTCTACGCTGG	50.0	50.0	57.3	57.8	574-575 bp	1.0	77.0