Hotspot

psbM-trnD-GUC

LSC · 29373-30175 · 803 bp

Back to Agriophyllum

Pi 0.112

MarkerSeek score 90.50

Primer available yes

Label psbM-trnD-GUC

Feature Metadata

label_name: psbM-trnD-GUC
feature_type: igs
region: LSC
length_bp: 803
variable_sites: 88
pi: 0.112
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 90.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-trnD-GUC_p1	TCATTTTGGCTGACAGTTTT	TCAATTGGTTAGAGCACCGC	35.0	50.0	53.9	58.6	961-967 bp	1.0	51.2
psbM-trnD-GUC_p2	TCATTTTGGCTGACAGTTTTTACA	TCAATTGGTTAGAGCACCGC	33.3	50.0	57.8	58.6	961-967 bp	1.0	50.4
psbM-trnD-GUC_p3	TCATTTTGGCTGACAGTTTTT	TCAATTGGTTAGAGCACCGC	33.3	50.0	54.8	58.6	961-967 bp	1.0	50.2
psbM-trnD-GUC_p4	AATCATTTTGGCTGACAGTT	TCAATTGGTTAGAGCACCGC	35.0	50.0	53.6	58.6	963-969 bp	1.0	49.2
psbM-trnD-GUC_p5	ATCATTTTGGCTGACAGTTT	TCAATTGGTTAGAGCACCGC	35.0	50.0	53.6	58.6	962-968 bp	1.0	49.2