Hotspot

atpI-rps2

LSC · 14898-15101 · 204 bp

Back to Agriophyllum

Pi 0.0294

MarkerSeek score 69.40

Primer available yes

Label atpI-rps2

Feature Metadata

label_name: atpI-rps2
feature_type: igs
region: LSC
length_bp: 204
variable_sites: 6
pi: 0.0294
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 69.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpI-rps2_p1	ATGTTGGCCTACTTCCACAC	ATTTGTGAAGGCCGCTCTAG	50.0	50.0	57.8	58.0	318-319 bp	1.0	79.7
atpI-rps2_p2	CGTGGATTTGGAAACCTCCT	ATTTGTGAAGGCCGCTCTAG	50.0	50.0	57.8	58.0	352-353 bp	1.0	79.5
atpI-rps2_p3	ATGTTGGCCTACTTCCACAC	TTTGCAATTTGTGAAGGCCG	50.0	45.0	57.8	58.1	324-325 bp	1.0	78.8
atpI-rps2_p4	CGTGGATTTGGAAACCTCCT	TTTGCAATTTGTGAAGGCCG	50.0	45.0	57.8	58.1	358-359 bp	1.0	78.6
atpI-rps2_p5	ATGTTGGCCTACTTCCACAC	TGAAGGCCGCTCTAGCTATA	50.0	50.0	57.8	57.7	313-314 bp	1.0	77.6