Hotspot

trnK-UUU-rps16

LSC · 4247-4922 · 676 bp

Back to Agriophyllum

Pi 0.0217
MarkerSeek score 69.00
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
676
variable_sites
14
pi
0.0217
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.95
markerseek_score
69.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 AAGCCGAGTACTCTACCGTT AAAGAGGGCGGAGGTTTTTA 50.0 45.0 58.2 57.0 732-764 bp 1.0 75.9
trnK-UUU-rps16_p2 AAGCCGAGTACTCTACCGTT AAGAGGGCGGAGGTTTTTAA 50.0 45.0 58.2 57.0 731-763 bp 1.0 75.9
trnK-UUU-rps16_p3 AAGCCGAGTACTCTACCGTT AGAGGGCGGAGGTTTTTAAA 50.0 45.0 58.2 57.0 730-762 bp 1.0 75.9
trnK-UUU-rps16_p4 AAGCCGAGTACTCTACCGTT AGGGCGGAGGTTTTTAAAGA 50.0 45.0 58.2 57.0 728-760 bp 1.0 75.9
trnK-UUU-rps16_p5 AAGCCGAGTACTCTACCGTT TAAAGAGGGCGGAGGTTTTT 50.0 45.0 58.2 57.0 733-765 bp 1.0 75.9