Hotspot

trnH-GUG-psbA

LSC · 76-372 · 297 bp

Back to Agriophyllum

Pi 0.0902
MarkerSeek score 74.80
Primer available yes
Label trnH-GUG-psbA

Feature Metadata

label_name
trnH-GUG-psbA
feature_type
igs
region
LSC
length_bp
297
variable_sites
24
pi
0.0902
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.90
markerseek_score
74.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnH-GUG-psbA_p1 GATCCACTTGGCTACATCCG CTGACGGTATGCCTCTAGGA 55.0 55.0 58.1 58.0 844-875 bp 1.0 84.7
trnH-GUG-psbA_p2 GATCCACTTGGCTACATCCG TCTGACGGTATGCCTCTAGG 55.0 55.0 58.1 58.0 845-876 bp 1.0 84.7
trnH-GUG-psbA_p3 CAATCCACTGCCTTGATCCA CTGACGGTATGCCTCTAGGA 50.0 55.0 57.9 58.0 858-889 bp 1.0 84.6
trnH-GUG-psbA_p4 CAATCCACTGCCTTGATCCA TCTGACGGTATGCCTCTAGG 50.0 55.0 57.9 58.0 859-890 bp 1.0 84.6
trnH-GUG-psbA_p5 AATCCACTGCCTTGATCCAC CTGACGGTATGCCTCTAGGA 50.0 55.0 57.9 58.0 857-888 bp 1.0 84.6