Hotspot

ndhF-rpl32

SSC · 112791-113927 · 1137 bp

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Pi 0.00235

MarkerSeek score 56.00

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 1137
variable_sites: 8
pi: 0.00235
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 56.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	AGTCCCGCTCCTATTAGCAT	TGCTGCCCAATACCCTTTAC	50.0	50.0	58.0	57.9	1280-1300 bp	1.0	80.5
ndhF-rpl32_p2	AGTCCCGCTCCTATTAGCAT	AAAAGCTTTCAATGCTGCCC	50.0	45.0	58.0	57.8	1292-1312 bp	1.0	80.2
ndhF-rpl32_p3	AAAGTAGAAGTCCCGCTCCT	TGCTGCCCAATACCCTTTAC	50.0	50.0	58.1	57.9	1288-1308 bp	1.0	80.1
ndhF-rpl32_p4	AAAGTAGAAGTCCCGCTCCT	AAAAGCTTTCAATGCTGCCC	50.0	45.0	58.1	57.8	1300-1320 bp	1.0	79.8
ndhF-rpl32_p5	AGTCCCGCTCCTATTAGCAT	AATGCTGCCCAATACCCTTT	50.0	45.0	58.0	57.7	1282-1302 bp	1.0	79.6