Hotspot

rpl14-rpl16

LSC · 81374-81541 · 168 bp

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Pi 0.0129

MarkerSeek score 59.70

Primer available yes

Label rpl14-rpl16

Feature Metadata

label_name: rpl14-rpl16
feature_type: igs
region: LSC
length_bp: 168
variable_sites: 6
pi: 0.0129
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 59.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl14-rpl16_p1	CCGCTGTTATCCGCTACATT	TTATGAAATGAGCGGCGTGA	50.0	45.0	58.1	58.0	276-354 bp	1.0	84.3
rpl14-rpl16_p2	CCGCTGTTATCCGCTACATT	AGCGGCGTGACAGAAAATAT	50.0	45.0	58.1	58.0	266-344 bp	1.0	84.3
rpl14-rpl16_p3	TACACATCAATTCTCGGGCC	TTATGAAATGAGCGGCGTGA	50.0	45.0	57.7	58.0	296-374 bp	1.0	82.7
rpl14-rpl16_p4	TACACATCAATTCTCGGGCC	AGCGGCGTGACAGAAAATAT	50.0	45.0	57.7	58.0	286-364 bp	1.0	82.7
rpl14-rpl16_p5	CCGCTGTTATCCGCTACATT	GAGCGGCGTGACAGAAAATA	50.0	50.0	58.1	58.6	267-345 bp	1.0	79.3