Hotspot

rpl20-rps12

LSC · 69097-69901 · 805 bp

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Pi 0.00381
MarkerSeek score 55.60
Primer available yes
Label rpl20-rps12

Feature Metadata

label_name
rpl20-rps12
feature_type
igs
region
LSC
length_bp
805
variable_sites
8
pi
0.00381
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
55.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl20-rps12_p1 TTGTTCTACGTCTTCGAGCG GAAATCCCCTGCTCTTGGAG 50.0 55.0 58.0 58.0 908-909 bp 1.0 80.1
rpl20-rps12_p2 TCGAGCGATATACCCTCGTT GAAATCCCCTGCTCTTGGAG 50.0 55.0 58.1 58.0 895-896 bp 1.0 79.5
rpl20-rps12_p3 TTCGAGCGATATACCCTCGT GAAATCCCCTGCTCTTGGAG 50.0 55.0 58.1 58.0 896-897 bp 1.0 79.5
rpl20-rps12_p4 CGTCTTCGAGCGATATACCC GAAATCCCCTGCTCTTGGAG 55.0 55.0 57.8 58.0 900-901 bp 1.0 78.4
rpl20-rps12_p5 GTTCTACGTCTTCGAGCGAT GAAATCCCCTGCTCTTGGAG 50.0 55.0 57.5 58.0 906-907 bp 1.0 76.8