Hotspot

accD-psaI

LSC · 59074-59589 · 516 bp

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Pi 0.0173

MarkerSeek score 59.10

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 516
variable_sites: 19
pi: 0.0173
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 59.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	GAACCCAACGCTTACATTGC	CCATTGCAATTGCCGGAAAT	50.0	45.0	58.0	58.0	769-799 bp	1.0	83.8
accD-psaI_p2	ATTGATCCAGGGACTTGGGA	CCATTGCAATTGCCGGAAAT	50.0	45.0	58.0	58.0	1240-1270 bp	1.0	82.4
accD-psaI_p3	TTGATCCAGGGACTTGGGAT	CCATTGCAATTGCCGGAAAT	50.0	45.0	58.0	58.0	1239-1269 bp	1.0	82.4
accD-psaI_p4	ATTGATCCAGGGACTTGGGA	TTGCCGGAAATACTAAGCCC	50.0	50.0	58.0	58.0	1231-1261 bp	1.0	82.2
accD-psaI_p5	TTGATCCAGGGACTTGGGAT	TTGCCGGAAATACTAAGCCC	50.0	50.0	58.0	58.0	1230-1260 bp	1.0	82.2