Hotspot

trnL-UAA-trnF-GAA

LSC · 48402-48751 · 350 bp

Back to Agrimonia

Pi 0.00533

MarkerSeek score 58.50

Primer available yes

Label trnL-UAA-trnF-GAA

Feature Metadata

label_name: trnL-UAA-trnF-GAA
feature_type: igs
region: LSC
length_bp: 350
variable_sites: 5
pi: 0.00533
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 58.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-UAA-trnF-GAA_p1	AATCGTGAGGGTTCAAGTCC	TCTACCAGCTGAGCTATCCC	50.0	55.0	57.5	58.0	401-405 bp	1.0	77.8
trnL-UAA-trnF-GAA_p2	AATCGTGAGGGTTCAAGTCC	TTTGAACTGGTGACACGAGG	50.0	50.0	57.5	58.1	437-441 bp	1.0	77.2
trnL-UAA-trnF-GAA_p3	AATCGTGAGGGTTCAAGTCC	GTGCCAGGAACCAGATTTGA	50.0	50.0	57.5	58.1	452-456 bp	1.0	76.9
trnL-UAA-trnF-GAA_p4	AATCGTGAGGGTTCAAGTCC	TCAGTCCTCTGCTCTACCAG	50.0	55.0	57.5	57.9	413-417 bp	1.0	76.7
trnL-UAA-trnF-GAA_p5	AATCGTGAGGGTTCAAGTCC	CATGTGCCAGGAACCAGATT	50.0	50.0	57.5	58.2	455-459 bp	1.0	76.4