Hotspot

petN-psbM

LSC · 28386-29654 · 1269 bp

Back to Agrimonia

Pi 0.00494

MarkerSeek score 62.80

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 1269
variable_sites: 15
pi: 0.00494
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 62.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	AGTAAATCTCGCTTGGGCTG	AAAACAGTCAGCCAAGGTGA	50.0	45.0	58.0	57.6	1359-1375 bp	1.0	78.1
petN-psbM_p2	AGTATGGGGAAGGAGTGGAC	AAAACAGTCAGCCAAGGTGA	55.0	45.0	58.1	57.6	1302-1318 bp	1.0	77.5
petN-psbM_p3	GTATGGGGAAGGAGTGGACT	AAAACAGTCAGCCAAGGTGA	55.0	45.0	58.1	57.6	1301-1317 bp	1.0	77.5
petN-psbM_p4	TATGGGGAAGGAGTGGACTC	AAAACAGTCAGCCAAGGTGA	55.0	45.0	57.8	57.6	1300-1316 bp	1.0	77.0
petN-psbM_p5	GGGGAAGGAGTGGACTCTAG	AAAACAGTCAGCCAAGGTGA	60.0	45.0	58.2	57.6	1297-1313 bp	1.0	76.7