Hotspot

trnC-GCA-petN

LSC · 27512-28295 · 784 bp

Back to Agrimonia

Pi 0.00642

MarkerSeek score 53.10

Primer available yes

Label trnC-GCA-petN

Feature Metadata

label_name: trnC-GCA-petN
feature_type: igs
region: LSC
length_bp: 784
variable_sites: 13
pi: 0.00642
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 53.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-petN_p1	GATTTGGTACCGTTTTGGCG	CAGCCCAAGCGAGATTTACT	50.0	50.0	58.0	58.0	891-930 bp	1.0	81.4
trnC-GCA-petN_p2	TTTCCCCAGTTCAAATCCGG	CAGCCCAAGCGAGATTTACT	50.0	50.0	58.1	58.0	833-872 bp	1.0	81.1
trnC-GCA-petN_p3	GATTTGGTACCGTTTTGGCG	AGTCCACTCCTTCCCCATAC	50.0	55.0	58.0	58.1	949-988 bp	1.0	80.6
trnC-GCA-petN_p4	GATTTGGTACCGTTTTGGCG	GTCCACTCCTTCCCCATACT	50.0	55.0	58.0	58.1	948-987 bp	1.0	80.6
trnC-GCA-petN_p5	TTTCCCCAGTTCAAATCCGG	GTCCACTCCTTCCCCATACT	50.0	55.0	58.1	58.1	890-929 bp	1.0	80.3