Hotspot

rpoB-trnC-GCA

LSC · 26433-27440 · 1008 bp

Back to Agrimonia

Pi 0.00834

MarkerSeek score 59.40

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1008
variable_sites: 18
pi: 0.00834
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	TCATTTTCACCCCCAAGCAT	CCGGATTTGAACTGGGGAAA	45.0	50.0	57.7	58.1	1085-1090 bp	1.0	78.7
rpoB-trnC-GCA_p2	ACTCATTTTCACCCCCAAGC	CCGGATTTGAACTGGGGAAA	50.0	50.0	58.4	58.1	1087-1092 bp	1.0	78.4
rpoB-trnC-GCA_p3	CTCATTTTCACCCCCAAGCA	CCGGATTTGAACTGGGGAAA	50.0	50.0	58.4	58.1	1086-1091 bp	1.0	78.4
rpoB-trnC-GCA_p4	TCATTTTCACCCCCAAGCAT	TATCAGCAAAACAGGAGGGC	45.0	50.0	57.7	57.9	1138-1143 bp	1.0	78.3
rpoB-trnC-GCA_p5	ACTCATTTTCACCCCCAAGC	TATCAGCAAAACAGGAGGGC	50.0	50.0	58.4	57.9	1140-1145 bp	1.0	77.9