Hotspot

trnS-GCU-trnG-GCC

LSC · 8442-9042 · 601 bp

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Pi 0.00833
MarkerSeek score 55.60
Primer available yes
Label trnS-GCU-trnG-GCC

Feature Metadata

label_name
trnS-GCU-trnG-GCC
feature_type
igs
region
LSC
length_bp
601
variable_sites
11
pi
0.00833
unique_haplotype_count
5
primer_available
yes
alignment_reliability
0.97
markerseek_score
55.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GCU-trnG-GCC_p1 CAATCCGACGCTTTAGTCCA AATCAAACCGAAAGACCCTT 50.0 40.0 58.0 55.1 696-736 bp 1.0 60.9
trnS-GCU-trnG-GCC_p2 CAATCCGACGCTTTAGTCCA ATCAAACCGAAAGACCCTTT 50.0 40.0 58.0 55.1 695-735 bp 1.0 60.9
trnS-GCU-trnG-GCC_p3 AATCCGACGCTTTAGTCCAC ATCAAACCGAAAGACCCTTT 50.0 40.0 58.0 55.1 694-734 bp 1.0 60.9
trnS-GCU-trnG-GCC_p4 AATCCGACGCTTTAGTCCAC AATCAAACCGAAAGACCCTT 50.0 40.0 58.0 55.1 695-735 bp 1.0 60.8
trnS-GCU-trnG-GCC_p5 ACGGAAAGAGAGGGATTCGA ATCAAACCGAAAGACCCTTT 50.0 40.0 58.2 55.1 749-789 bp 1.0 59.4