Hotspot

trnH-GUG-psbA

LSC · 106-437 · 332 bp

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Pi 0.0135

MarkerSeek score 56.40

Primer available yes

Label trnH-GUG-psbA

Feature Metadata

label_name: trnH-GUG-psbA
feature_type: igs
region: LSC
length_bp: 332
variable_sites: 12
pi: 0.0135
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 56.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	GATCCACTTGGCTACATCCG	TTTCCCTCTAGACCTAGCGG	55.0	55.0	58.1	57.9	381-402 bp	1.0	84.9
trnH-GUG-psbA_p2	CAATCCACTGCCTTGATCCA	TTTCCCTCTAGACCTAGCGG	50.0	55.0	57.9	57.9	395-416 bp	1.0	84.8
trnH-GUG-psbA_p3	AATCCACTGCCTTGATCCAC	TTTCCCTCTAGACCTAGCGG	50.0	55.0	57.9	57.9	394-415 bp	1.0	84.8
trnH-GUG-psbA_p4	ACAATCCACTGCCTTGATCC	TTTCCCTCTAGACCTAGCGG	50.0	55.0	57.9	57.9	396-417 bp	1.0	84.7
trnH-GUG-psbA_p5	ATTCATTGATGGGCGAACGA	TTTCCCTCTAGACCTAGCGG	45.0	55.0	58.0	57.9	466 bp	0.166667	45.8