Hotspot

rps16

LSC · 5305-6863 · 1559 bp

Back to Aglaonema

Pi 0.0184

MarkerSeek score 59.80

Primer available yes

Label rps16

Feature Metadata

label_name: rps16
feature_type: gene
region: LSC
length_bp: 1559
variable_sites: 27
pi: 0.0184
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 59.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16_p1	AAGAAAACGAGGGCAGATCC	ATCAATTCCATCCCGGTTCG	50.0	50.0	57.9	58.0	2153-2209 bp	1.0	83.9
rps16_p2	AAGAAAACGAGGGCAGATCC	CAATTCCATCCCGGTTCGAA	50.0	50.0	57.9	58.3	2151-2207 bp	1.0	82.1
rps16_p3	AGACTTGTGTTGGATTGGCA	ATCAATTCCATCCCGGTTCG	45.0	50.0	57.6	58.0	1868-1948 bp	1.0	82.0
rps16_p4	AATGCACGATCCCACCAAAA	ATCAATTCCATCCCGGTTCG	45.0	50.0	58.4	58.0	2250-2305 bp	1.0	81.8
rps16_p5	ATGCACGATCCCACCAAAAA	ATCAATTCCATCCCGGTTCG	45.0	50.0	58.4	58.0	2249-2304 bp	1.0	81.8