Hotspot

ndhB-trnL-CAA

IRa · 153823-154886 · 1064 bp

Back to Aglaonema

Pi 0.0039

MarkerSeek score 64.40

Primer available yes

Label ndhB-trnL-CAA

Feature Metadata

label_name: ndhB-trnL-CAA
feature_type: igs
region: IRa
length_bp: 1064
variable_sites: 4
pi: 0.0039
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 64.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhB-trnL-CAA_p1	TGCAATTGCTCAGGATACCC	GTCTCGCGTGTCTACCATTT	50.0	50.0	57.9	58.0	1124-1126 bp	1.0	82.5
ndhB-trnL-CAA_p2	TGCAATTGCTCAGGATACCC	CTCGCGTGTCTACCATTTCA	50.0	50.0	57.9	58.0	1122-1124 bp	1.0	82.5
ndhB-trnL-CAA_p3	TGCAATTGCTCAGGATACCC	GCGTGTCTACCATTTCACCA	50.0	50.0	57.9	58.2	1119-1121 bp	1.0	81.0
ndhB-trnL-CAA_p4	TGCAATTGCTCAGGATACCC	TCTCGCGTGTCTACCATTTC	50.0	50.0	57.9	57.7	1123-1125 bp	1.0	80.3
ndhB-trnL-CAA_p5	TGCAATTGCTCAGGATACCC	TCGCGTGTCTACCATTTCAC	50.0	50.0	57.9	58.3	1121-1123 bp	1.0	80.2