Hotspot

accD-psaI

LSC · 64291-65398 · 1108 bp

Back to Aglaonema

Pi 0.0163

MarkerSeek score 72.60

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 1108
variable_sites: 17
pi: 0.0163
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 72.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	ATTTCAGCTCCACGGTTTCC	CCATTGCAATTGCCGGAAAT	50.0	45.0	58.5	58.0	1156-1221 bp	1.0	81.2
accD-psaI_p2	ATTTCAGCTCCACGGTTTCC	AAGAAGCCATTGCAATTGCC	50.0	45.0	58.5	57.9	1162-1227 bp	1.0	80.6
accD-psaI_p3	CACGGTTTCCTTCCCTTGAC	CCATTGCAATTGCCGGAAAT	55.0	45.0	58.8	58.0	1146-1211 bp	1.0	78.8
accD-psaI_p4	CACGGTTTCCTTCCCTTGAC	AAGAAGCCATTGCAATTGCC	55.0	45.0	58.8	57.9	1152-1217 bp	1.0	78.2
accD-psaI_p5	ACGGTTTCCTTCCCTTGACT	CCATTGCAATTGCCGGAAAT	50.0	45.0	58.9	58.0	1145-1210 bp	1.0	78.0